Everest Biolabs
Ascent Instrument는 Apex SEC(Size Exclusion Column) 컬럼을 최대 8개까지 장착하여 자동으로 Isolation을 해주는 시스템으로서 세포밖소포(Extracellular Vesicle) 및 엑소좀(Exosome)의 손쉽고 순도 높은 분리가 가능합니다.
Summit Instrument는 48 wells Altitude plate를 장착하여 샘플 로딩(Sample Loading) 및 세포밖소포의 분리 정제(EV isolation)와 농축(Concentration)을 전자동으로 수행하는 시스템입니다.
Atlas ELISA Kit 및 Atlas ELISA Reader를 사용하면 50μL 샘플만으로 한 번에 8개 wells 또는 96 well plate 전체를 한 번에 분석할 수 있습니다.
Apex SEC Plates
Apex 4B plate
Apex 6B plate
Altitude Concentration Plates
Apex SEC Columns
Apex 4B
Apex 6B
Apex MM
Apex mini
Atlas ELISA Kits
Atlas Human EV ELISA Kit
Atlas Lumi Homebrew Kit
Atlas Lumi Human EV ELISA Kit
Apex SEC Columns & Plates
제품 브로슈어 다운로드Apex SEC columns
High performance isolation
Reproducible performance < 10% CV
Sample volume 0.1-1.0 mL
Available with SEC and multi-mode resins
Validated in culture, plasma, urine, serum & CSF
Compatible with Ascent and Summit
Apex 4B
Apex 6B
Apex MM
Apex mini
Apex 4B
1Optimized with 35 nm pore size gel for lowest protein co-elution
2Ideal for unbiased downstream analysis, including proteomics and RNA-sequencing.
3Reusable up to 5 times
Apex 6B
1Optimized with 20 nm pore size gel for the highest total EV recovery.
2Ideal for targeted downstream analysis, such as immunoassays, label-based imaging assays, label-based flow assays, western blots, and pull-down using a specific surface marker.
3Reusable up to 5 times
Apex MM
1Optimized for maximum EV purity in plasma/serum samples.
2Ideal for unbiased assays like Mass Spec and flow cytometry.
3Reusable up to 3 times
Apex mini
1Optimized for small sample volumes (0.1 ~ 0.3 mL)
2Similar performance to Apex MM
3Reusable up to 3 times
| Sample Type | Analysis Type | Recommended Column |
| Plasma, Serum, or Cell Culture Media (with FBS/Lipoproteins) | Single Particle (e.g. NTA/Nanoflow) | Apex MM |
| Targeted Assays (e.g. ELISA/WB/ONI/Leprechaun) |
Apex 4B | |
| Untargeted Assays (e.g. Proteomics) |
Apex MM | |
| Cell Culture Media (Serum-free Media) | Most applications | Apex 6B |
| CSF | ||
| Urine |
Apex SEC Working Principle
Size-exclusion chromatography (SEC) gently separates extracellular vesicles (EVs) by size as a sample passes through a column packed with porous beads. Large particles that cannot enter the pores elute first, while smaller molecules (e.g., secreted proteins) enter the pores and travel a longer path, causing them to elute later. This creates a key trade-off: smaller pore sizes increase EV yield but allow more contaminants to co-elute, whereas larger pores improve purity by retaining more mid-sized impurities but reduce EV recovery.
Apex SEC plates
Scale your isolation
Get the same results as Apex SEC columns in a plate format.
Compatible with the Summit instrument
Apex SEC Columns
1. Apex 4B & 6B
Apex 4B SEC Columns
High performance isolation
Reproducible performance < 10% CV
Available with multiple gel types: 4B (high-purity)
Sample volume 0.5-1.0 mL
SKUs
- APEX-4B-8 (8-pack)
- APEX-4B-4 (4-pack)
Apex 6B SEC Columns
High performance isolation
Reproducible performance < 10% CV
Available with multiple gel types: 6B (high-yield)
Sample volume 0.5-1.0 mL
SKUs
- APEX-6B-8 (8-pack)
- APEX-6B-4 (4-pack)
How do I apply Apex columns to my research?
◼︎ Apex 4B: Optimized for balanced purity and yield in plasma—well-suited for targeted assays (immunoassays, imaging, Western blot, pull-downs).
◼︎ Apex 6B: Optimized for maximum EV recovery—best for samples with low-containments like serum-free cell culture media, CSF, and urine.
◼︎ integration note: Apex SEC columns work with the Ascent instrument for improved reproducibility and throughput. Apex SEC plates are designed for full automation on the Summit instrument.
Apex 4B and 6B performance with plasma
◼︎ Apex 4B: Optimized for high purity and lowest protein co-elution (ideal for biofluids)
◼︎ Apex 6B: Optimized for highest EV yield (ideal for cell cultures)
| Column Type | Purity | Yield | Pore-Size |
|---|---|---|---|
| Apex 6B | 1.0 | 1.0 | 20 nm |
| Apex 4B | 5.0 | 0.8 | 35 nm |
| Competitor | 5.0 | 0.3 | 70 nm |
Definitions :
Yield = EVs in fractions 1–3, normalized to Apex 6B
Purity = Yield/HSA, normalized to Apex 6B
Apex maintains performance with reuse
Apex maintains performance and drip speed after 5 reuses. Columns were tested by running 0.5 mL of human plasma sample, and pooling fractions 1–3, which represent the EV fractions.
Columns were washed with 4 mL of NaOH, followed by 17 mL of 1X PBS between reuses. EVs and HSA were quantified using the Atlas ELISA
Apex 4B and 6B isolate the same EVs
EV composition and size comparison. Super resolution imaging (ONI) and size comparison by NTA and TEM show that Apex 4B and 6B purify EVs with the same tetraspanin composition and size.
Cell culture Sample
NTA/TEM
ONI
Plasma Sample
NTA/TEM
ONI
Technical information
The Apex size exclusion column (SEC) purifies extracellular vesicles (EVs) from biological fluids such as plasma, serum, urine, cell culture media, or cerebrospinal fluid (CSF).
Everest Biolabs Atlas EV and human serum albumin (HSA) ELISA kits can be used to optimize EV yield and purity during fraction collection.
| Column format | Column | Plate | mini |
| Column type | MM / 4B / 6B | 4B / 6B | MM |
| Input sample volume | 0.5 - 1.0 mL | 0.5 - 1.0 mL | 0.1 - 0.3 mL |
| Column volume | 9.0 mL | 9.0 mL | 3.0 mL |
| Column reproducibility | 10% CV | ||
| Sample types | plasma, serum, urine, CSF, cell culture media | ||
| Resin types | 4% or 6 % cross linked agarose beads, cross linked agarose plus multi-mode | ||
| Exclusion limit | 35 nm or 20 nm | ||
2. Apex MM & mini
Apex MM Columns
Superior EV purity
Reproducible performance < 10% CV
Combines SEC and multi-mode resins
Sample volume 0.5-1.0 mL
SKUs
- APEX-MM-8 (8-pack)
- APEX-MM-4 (4-pack)
Apex mini Columns
Superior EV purity
Reproducible performance < 10% CV
Availabe in MM format
Sample volume 0.1-0.3 mL
SKUs
- APEX-MINI-MM (16-pack)
Apex MM: More than just SEC
By combining SEC with a multi-mode (MM) resin, Apex MM columns significantly enhance EV purity by removing more lipoproteins than SEC alone.
By providing superior EV purity, maintaining optimal yield without introducing size biases, and preserving EV functionality, Apex MM columns significantly enhance biomarker discovery and therapeutic research applications
Optimized for maximum EV purity in plasma/serum samples—optimal for unbiased assays like mass spec and flow cytometry.
Apex MM performance with plasma
◼︎ Apex MM: Optimized for maximum EV purity in plasma/serum samples—optimal for unbiased assays like mass spec and flow cytometry.
| Column Type | Yield | Purity (HSA) | Purity (ApoA1) | Purity (ApoB-100) | Pore Size |
|---|---|---|---|---|---|
| Apex MM | 1.5 | 2.2 | 1.1 | 1.8 | < 50 nm |
| Competitor | 1.0 | 1.0 | 1.0 | 1.0 | 70 nm |
Definitions :
Yield = EVs in fractions 1–3, normalized to Competitor
Purity (HSA) = Yield/HSA, normalized to Competitor
Purity (ApoA1) = Yield/ApoA1, normalized to Competitor
Purity (ApoB-100) = Yield/ApoB-100, normalized to Competitor
Apex mini, smaller size, same performance
50% more EVs and 2x purity
Optimized for small sample volumes (0.1 – 0.3 mL)
Similar performance to Apex MM
Easy-open cap
Works on the Ascent instrument
Plasma Sample
NTA
ONI
Technical information
The Apex MM column purifies extracellular vesicles (EVs) from biological fluids such as plasma, serum, urine, cell culture media, or cerebrospinal fluid (CSF).
Everest Biolabs Atlas EV and human serum albumin (HSA) ELISA kits can be used to optimize EV yield and purity during fraction collection.
| Column format | Column | mini |
| Column type | MM / 4B / 6B | MM |
| Input sample volume | 0.5 - 1.0 mL | 0.1 - 0.3 mL |
| Column volume | 8.75 mL | 3.5 mL |
| Column reproducibility | 10% CV | |
| Sample types | plasma, serum, urine, CSF, cell culture media | |
| Resin types | cross-linked agarose plus multi-mode | |
3. Apex SEC Plates
Apex SEC Plates
High performance isolation, at scale
Reproducible performance < 10% CV
Available with multiple gel types : 4B (high-purity), 6B (high-yield)
Sample volume 0.5-1.0 mL
Optimized for the Summit instrument
SKUs
- APEX-4B-PLATE24 (4B plate - 24 wells)
- APEX-6B-PLATE24 (6B plate - 24 wells)
Apex built for automation at any scale
Run Apex single columns or plates depending on your project needs
Single columns and plate designed to be interchangeable
SEC single columns compatible with Ascent and Summit instrument
SEC Plates are compatible with Summit instrument
Ascent Instrument
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Ascent Instrument
Automated, high-throughput EV isolation with SEC columns
Run up to 8 columns in parallel
Collect up to 12 fractions per column
Wash up to 8 columns
Compatible with different column types
Programmable fraction collection sequences
Programmable fraction collection
Optimize your fraction collection for your application.
You may adjust these values for your application to work with different column types, between 1 and 8 columns, wash volumes, number of fractions to collect, and the fraction volume.
Apex SEC Columns
High performance isolation
Reproducible performance < 10% CV
Sample volume 0.1-1.0 mL
Available with SEC and multi-mode resins
Validated in culture, plasma, urine, serum & CSF
Compatible with Ascent and Summit
Watch Ascent in action
1. Set run parameters
2. Load columns and tubes
3. Wash
4. Add sample
5. Fractionate
Apex column reproducibility
The Apex SEC columns are optimized for a consistent drip speed resulting in reproducible EV elution profile.
Pairing the Apex columns with the Ascent and Summit instruments further minimizes variability in EV elution across columns.
Technical information
The Ascent instrument is an automated high-throughput fraction collector for isolation of extracellular vesicles (EVs) with SEC columns.
It can operate up to 8 columns simultaneously and is compatible with Apex columns as well as other brands. The programmable fraction collection allows you to optimize collection for any application.
| SEC column capability | Apex 4B Apex 6B Apex MM Pack your own |
| Compatible with qEV™ SEC columns (qEV™ is a trademark of Izon Science®; not affiliated). |
qEVoriginal qEV1 qEVsingle |
| Dimensions (WxDxH) | 397mm x 364mm x 331mm 15-5/8” x 14-5/8” x 13” |
| Weight | 13.6 Kg / 30.0 lbs. |
| Input Power | 100~240 VAC (50-60Hz) |
| Power Supply Unit | 24 Vdc, 2.1A, 50W |
| Environmental Temperature | 10°C -24°C (50°F-75°F) |
| Relative Humidity | 30-80% RH |
| Bottle Capacity | 1000mL |
| Connectivity | USB-C (for software updates) |
| Chemical Compatibility | PBS, DI, 70% ethanol, dilute bleach |
Summit Instrument
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Summit Instrument
EV isolation and concentration for high-capacity labs
Process up to 48 samples in parallel
Fully automated, including sample loading
Collect 1-to-3 fractions per sample
Plate-based ultrafiltration for concentration
Apex SEC columns and Plates
Isolation that matches your purity and throughput needs
Reproducible performance < 10% CV
Available with multiple gel types:
- 4B (high-purity)
- 6B (high-yield)
- MM (high-lipoprotein purity)
Sample volume 0.1-1.0 mL
Concentrate 4 to 48 samples
Altitude concentration plates for the Summit
Uses ultrafiltration membranes to concentrate EV fractions directly in a 24-well format
Designed to balance high recovery with reduction in liquid volume (down to 100 uL)
Direct collection of SEC fractions into Altitude UF plates during automated runs
Automate your workflow
Ascent vs. Summit: Choose Your Ideal Workflow
Compare features, throughput, and flexibility to find the best fit for your research needs.
 |
 |
|
Ascent |
Summit |
|
Best For |
Flexible sample prep | Large cohorts and standardized workflows |
Throughput Focus |
Moderate | High |
SEC Format |
Columns | Plates or Columns |
Sample Capacity |
1–8 samples | 4–48 samples |
Automation Level |
Manual sample loading required | Fully automated operation |
Fraction Collection |
1–12 fractions per sample | 1–3 fractions per sample |
Concentration Handling |
Performed externally | Integrated capability |
Footprint |
Compact | Larger, designed for scale |
Summit Instrument Specifications
| Units | Imperial | Metric |
| Dimensions (WxDxH) | 36” x 27” x 30” | 91 x 69 x 76 cm |
| Weight | 133 lbs. | 50 Kg |
| Bottle capacity | 1 U.S. gallons | 4 L |
| User Interface | 5.6” | 14.3mm |
| Environmental temperature | 50°F-75°F | 10°C -24°C |
| Relative humidity | 30−80% RH | |
| Input power | 100~240 VAC (50−60 Hz) | |
| Power supply unit | 24 VDC, 5 A, 120 W | |
| Connectivity | USB 3.1 | |
Atlas ELISA kit
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Atlas Human EV ELISA Kit
Easy and reliable concentration assay
Human EV ELISA for EV quantification
Run direct from biofluid or SEC fractions
Run 8 wells at a time or the full plate
Only 50 µL of sample – no need to concentrate
SKUs
- ATLA-EV
Atlas Lumi Human EV ELISA Kit
High-sensitivity, high-dynamic range ELISA for EV biomarker quantification
Chemilumiescene with large dynamic range
Human EV ELISA for EV quantification
Run direct from biofluid or SEC fractions
Run 8 wells at a time or the full plate
Only 50 µL of sample – no need to concentrate
SKUs
- ATLAS-LUMI-EV
Atlas Lumi Homebrew Kit
High-sensitivity, high-dynamic range ELISA for EV biomarker quantification
Chemilumiescene with large dynamic range
Includes custom conjugation kit for use with custom targets
Human EV ELISA for EV quantification
Run direct from biofluid or SEC fractions
Run 8 wells at a time or the full plate
Only 50 µL of sample – no need to concentrate
SKUs
- ATLAS-LUMI-HOMEBREW
Human EV ELISA
Validated in:
Plasma
Serum
CSF
Urine
Cell culture media
The Atlas EV ELISA is available in two formats:
◼︎ Absorbance ELISA: Simple and reliable EV yield and purity assessment using standard plate readers.
◼︎ Chemiluminescence (Lumi) ELISA: High sensitivity, wide dynamic range, and customizable capture (Homebrew options) for profiling disease-relevant markers.
| Atlas Human EV | Atlas Lumi Human EV | |
| Dynamic range | 2 logs | 4 logs |
| Limit of detection | 1e8 | 1e7 |
| Reader compatibility | Absorbance | Chemiluminescence |
| Custom targets |
Incubation
Add to the well plate
Dilute sample
Antibody mix
Wash Step
5X with Wash Buffer
Signal Generation
Add to the well-plate
100 uL TMB solution (5-10min)
100 uL Stop Solution
Signal Readout
Measure absorbance with plate reader
Human EV ELISA
Validated in :
Plasma
Serum
CSF
Urine
Cell culture media
Atlas Lumi ELISA
High-sensitivity, high-dynamic range ELISA for EV biomarker quantification
Technical information
The Atlas Human EV ELISA is an immunoassay to measure intact extracellular vesicles (EVs) in plasma, serum, urine, cell culture media, or cerebrospinal fluid (CSF).
This kit contains enough reagents for 96 wells.
| Absorbance | Chemiluminescence (Lumi) | |
| Reactive species | Human | Human |
| Well format | 8 x 12 strips | 8 x 12 strips |
| Dynamic range | 1e8 – 1e10 particles / mL | 1e7 - 1e11 particles / mL |
| Assay reproducibility (CV) | <10% | <10% |
| Limit of detection (LOD) | 1e8 particles / mL | 1e7 particles / mL |
| Lower limit of quantification (LLOQ) | 3.5e8 particles / mL | 1.4e7 particles / mL |
| Sample types | Plasma Serum Urine CSF Cell culture media |
Plasma Serum Urine CSF Cell culture media |
Atlas ELISA Reader
Compact, high-performance absorbance reader
Measure 96 wells at once
Get results in seconds
Space-saving format