위드인스트루먼트

Particle Metrix

제품소개

Particle Metrix

Particle Metrix

C-NTA 나노입자추적분석기 ZetaView^Ⓡ

나노 입자가 브라운 운동이나 전기 영동에 의해 움직이는 궤적을 추적하여 Stokes-Einstein equation에 의해 입자의 크기(Size)를 분석하고, mL당 입자의 개수(Concentration)와 제타 전위(Zeta Potential)를 분석하는 시스템입니다.
형광 필터를 통해 Fluorescence Particles의 Size와 Concentration을 분석함으로써 전체 입자 중 형광 입자의 비율을 계산할 수 있습니다.

제품 특징

1. 기기 한 대에 4개 레이저 동시 장착 가능 (QUATT)
2. 1 Click Auto Focusing - 사용자간 편차 없음
3. 1 Click 레이저 변경 및 형광 모드 전환
4. 11 positions / min. 스캐닝 분석
5. 제타 전위(Zeta Potential) 분석 가능
6. Intensity 차이로 한 샘플 내 혼합 입자 크기 구분 가능
7. Pump를 통한 Sample Cell 자동 세척

모델 추천구성

MONO ZetaView^Ⓡ(PMX-130)

· Laser 1개 장착
· 자동 형광 필터
· 동일 샘플 내 1개의 형광 입자 분석 가능

제품 상세 보기

TWIN ZetaView^Ⓡ(PMX-230)

· 한 기기에 2개의 Laser 장착
· 자동 형광 필터
· 동일 샘플 내 2개의 형광 입자 분석 가능

제품 상세 보기

QUATT ZetaView^Ⓡ(PMX-430)

· 한 장비에 Laser 4개 동시 장착
· 자동 형광 필터
· 동일 샘플 내 4개의 형광 입자 분석 가능

제품 상세 보기

제품 브로슈어 다운로드

형광 분석


Laser	Dyes	Photostability
405 nm	Alexa® Fluor 405	+
	Alexa® Fluor 430	++
	Quantum dots	+++
	Cell tracker violet	++
488 nm	Alexa® Fluor 488	+++
	Atto 488	+++
	PE, PE-Cy5	++
	FITC	---
	Rhodamine B	+
	Calcein AM	+
	CMG, PKH67, DIO	+++/++/++
	GFP, eGFP	+/++


Laser	Dyes	Photostability
520 nm	Alexa® Fluor 546	+++
	DyLight^TM 550	+++
	Alexa Fluor 555	+++
	PE, PKH26 / PE-Cy5	+/++
	Cy3	++
	CMO	+++
	RFP	+++
640 nm/ 660 nm	Alexa® Fluor 647	++
	APC	++
	Cy5	+++
	CMDR, DIL	+++/++

상세 사양

General Features

General Features
Measurement principles	Precision-engineered motorized scanning Nanoparticle Tracking Analysis (NTA) instrument for tracking the movement of individual visualized nanoparticles in suspension Real-time visualization of Brownian Motion and electrophoretic mobility, for measuring size, concentration and zeta potential in scattering and fluorescence modes. Four simultaneous aligned and software-controlled lasers for fluorescence measurements Software controlled emission filter changer for quick changes between the fluorescence measurements Fast scanning to acquire and analyze typically 1000 particles in ~ 1 minute Software-controlled pumps for flushing and sample sub-dosing
Samples	Nanoparticles suspended in polar liquids (e.g. water, alcohols) for size, concentration, fluorescence and zeta potential studies

Hardware

Hardware
Optical layout	90° laser scattering video microscope with x10 magnification Automated alignment and focusing of laser and microscope"
Camera	High sensitive CMOS camera 640 x 480 pixels Variable frame rate from 1 to 60 Hz for optimum resolution and fast acquisition"
Fluorescence Filter sets	Software controlled, automated filter wheel with four long-pass fluorescence emission filters(LWP) with cut-off at 430 nm / 500 nm / 550 nm / 680 nm [QUATT] Bandpass filter available on request
Cell Assembly	Z-NTA slide-in assembly for size, concentration and dual fluorescence measurements plus zeta potential experiments in aqueous and organic solvents with pumps for 2 different liquids/buffers – for rinsing and sub-dosing experiments, electrical field sensing
Cleaning	Cell cleaning recommended weekly – cell resistant to >1000 brush cleanings Cleaning of driver electrodes required after more than 1000 zeta potential runs Cleaning kit and basic replacement parts included in delivery
Temperature Range/Control	Working external temperature range: 5°C ~ 45°C Sample temperature control : Peltier temperature control from RTP -5°C ~ 55°C with dew-point sensing

Software

Software
Communication	Software provided on pre-configured PC, communication via Ethernet
Quality Control	Cell quality check, daily performance check, outlier control with automatic Grubbs statistical analysis of measurement data
Live Monitoring	Fully customizable SOPs for different samples / applications
Standard Operating Procedures (SOP)	Cell quality check, daily performance check, outlier control with automatic Grubbs statistical analysis of measurement data
Analysis and Reports	Data Analysis in scatter and / or fluorescence mode: particle size distribution profiles, concentration, overlays and averaging, scatter plots, zeta-potential distribution profiles, sub-population analysis Data export format: AVI, TXT, CSV, FCS PDF reports containing key results

Measurement Specifications

Measurement Specifications
Size/Concentration	Concentration range: 10⁵ ~ 10⁹ particles/ml Particle size: 10nm ~ 2000nm (dependent on sample and laser selection) Accuracy: ±5nm (for 100nm polystyrene latex) Reproducibility: ±2nm (for 100nm polystyrene latex)
Fluorescence	Concentration range: 10⁵ ~ 10⁹ particles/ml Particle size: 10nm ~ 2000nm (dependent on fluorescent dye and laser selection) Accuracy: ±5nm (for 100nm polystyrene latex) Reproducibility: ±2nm (for 100nm polystyrene latex)
Zeta Potential Reports	Working range: -500mV ~ +500mV Concentration range: 10⁶ ~10¹⁰ particles/ml Conductivity range: 3µS/cm ~ 15mS/cm Accuracy: ±4mV (for alumina zeta potential standard) Reproducibility: ±2mV (for alumina zeta-potential standard)
General	Minimum sample quantity: 500μl of sample at 10 particles/ml pH range: 1 ~ 13 Temperature: 5°C ~ 45°C (external temperature) Sample volume visualised and tracked by the camera for a single measurement: 11 x 3nL
Reference Materials	Nominal 100 nm reference suspension for size Two nominal 100 nm reference suspensions for fluorescence Nominal +50mV reference suspension for zeta potential

Dimensions

Dimensions
Physical	Footprint (W x D x H) : 20 x 30 x 25cm Weight : 8.5kg (main unit, PC extra) Shipping box with standard content : 48 x 62 x 63cm ; 22kg
Electrical	90~240V, 47~63Hz, 50VA

Warranty & Support


Warranty	1 year (sample cell glass excluded)
Service & Support	Reaction time: 48 hours Maintenance, service and IQ/OQ contracts available on demand Support via telephone, email and TeamViewer for trained users free of charge during warranty period Training courses for new users available on demand Special arrangements and specifications available on demand – quotation required